Search results for "Lithocholic Acid"

showing 10 items of 24 documents

Steroids aid in human decomposition fluid identification in soils of temporary mass graves from World War II

2020

Abstract Steroids are widely used for the detection of faecal matter and also – in recent years – for characterising human decomposition in the terrestrial environment. Until now it was not clear whether all commonly used faecal (i.e. 5β-stanols, 5β-stanones and bile acids) and tissue derived steroids (i.e. cholesterol, 5α-cholestanol and 5α-cholestanone) could reveal the presence of human decomposition products in temporary graves. In this study, soil was sampled from three putative mass graves where concentration camp prisoners were temporarily buried for 10 months at the end of World War II (1944 – 45). We hypothesised that soil from the putative temporary mass graves exhibits elevated c…

010506 paleontologyArcheologyLithocholic acid060102 archaeologyBile acidSoil testChemistrymedicine.drug_class06 humanities and the arts01 natural sciencesDecompositionCoprostanolchemistry.chemical_compoundEnvironmental chemistryFaecal matterSoil watermedicine0601 history and archaeologyddc:9300105 earth and related environmental sciencesJournal of Archaeological Science: Reports
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Anhydrobiosis in Yeasts: Changes in Mitochondrial Membranes Improve the Resistance of Saccharomyces cerevisiae Cells to Dehydration–Rehydration

2019

Anhydrobiosis is a unique state of live organisms in which their metabolism is temporary reversibly suspended as the result of strong dehydration of their cells. This state is widely used currently during large-capacity production of active dry baker&rsquo

0106 biological sciencesLithocholic acidSaccharomyces cerevisiaePlant ScienceMitochondrion01 natural sciencesBiochemistry Genetics and Molecular Biology (miscellaneous)<i>Saccharomyces cerevisiae</i>03 medical and health scienceschemistry.chemical_compound010608 biotechnologymedicinedehydration–rehydrationDehydrationCryptobiosis030304 developmental biologylcsh:TP500-6600303 health sciencesbiologyChemistryMetabolismlcsh:Fermentation industries. Beverages. Alcoholanhydrobiosismedicine.diseasebiology.organism_classificationYeastmitochondriaMembranelithocholic acidBiochemistryFood ScienceFermentation
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The Vitamin D Receptor Regulates Glycerolipid and Phospholipid Metabolism in Human Hepatocytes.

2020

The vitamin D receptor (VDR) must be relevant to liver lipid metabolism because VDR deficient mice are protected from hepatosteatosis. Therefore, our objective was to define the role of VDR on the overall lipid metabolism in human hepatocytes. We developed an adenoviral vector for human VDR and performed transcriptomic and metabolomic analyses of cultured human hepatocytes upon VDR activation by vitamin D (VitD). Twenty percent of the VDR responsive genes were related to lipid metabolism, including MOGAT1, LPGAT1, AGPAT2, and DGAT1 (glycerolipid metabolism)

0301 basic medicinemusculoskeletal diseasesmedicine.medical_specialtyVitaminesLithocholic acidMice Knockout ApoECèl·luleslcsh:QR1-502Phospholipidvitamin DBiochemistryCalcitriol receptorlcsh:MicrobiologyArticle03 medical and health scienceschemistry.chemical_compoundMice0302 clinical medicineBiomolèculesDownregulation and upregulationInternal medicinelipid metabolismmedicinepolycyclic compoundsAnimalsHumansvitamin D receptorMolecular BiologyPhospholipidsTriglyceridesPhosphatidylethanolaminedigestive oral and skin physiologyhuman hepatocytesLipid metabolismMetabolismHep G2 Cells030104 developmental biologyEndocrinologychemistryGene Expression Regulation030220 oncology & carcinogenesisHepatocytesReceptors Calcitriollipids (amino acids peptides and proteins)IntracellularBiomolecules
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Angiopoietin-Like Protein 8 Is a Novel Vitamin D Receptor Target Gene Involved in Nonalcoholic Fatty Liver Pathogenesis

2018

Hepatic vitamin D receptor (VDR) expression is increased in patients with nonalcoholic fatty liver (NAFL) and is required for liver steatosis in an NAFL mouse model. However, how hepatocyte VDR is involved in setting up steatosis remains unclear. The authors transduced human hepatocyte-derived cells with an adenoviral vector encoding human VDR and found that angiopoietin-like protein 8 (ANGPTL8) expression was increased upon VDR activation by vitamin D or lithocholic acid. The mRNA levels of hepatic VDR- and vitamin D-related genes [cytochrome P450 (CYP) 2R1, CYP27A1, and CYP3A4] were higher in NAFL patients compared with normal liver subjects. Noteworthy, hepatic ANGPTL8 mRNA and protein l…

AdultMale0301 basic medicinemedicine.medical_specialtyLithocholic acidPeptide HormonesFatty Acids NonesterifiedCalcitriol receptorPathology and Forensic Medicine03 medical and health scienceschemistry.chemical_compound0302 clinical medicineAngiopoietin-Like Protein 8Non-alcoholic Fatty Liver DiseaseInternal medicineCYP27A1medicineHumansInsulinCells CulturedTriglyceridesGene knockdownCYP3A4Fatty liverMiddle Agedmedicine.diseaseAngiopoietin-like Proteins030104 developmental biologymedicine.anatomical_structureEndocrinologyGene Expression RegulationchemistryCase-Control StudiesHepatocyteHepatocytesReceptors CalcitriolFemale030211 gastroenterology & hepatologySteatosisThe American Journal of Pathology
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Synthesis, characterization, and thermal behavior of steroidal dendrons

2004

A series of novel dendritic steroidal polyesters of first and second generation has been synthesized in convergent fashion by the use of 2,2-bis(hydroxymethyl)propionic acid as a repeating unit. The first- and second-generation hydroxy-functionalized dendrons with a variety of surface modifications were produced through the use of four bile acids: lithocholic acid (LCA), ursodeoxycholic acid (UDCA), deoxycholic acid (DCA), and cholic acid (CA). The thermal behavior of the steroidal dendrons was characterized by differential scanning calorimetry (DSC) and by thermogravimetric analysis (TGA). Finally, quantum chemical calculation methods were used to study the geometries of the dendrons and t…

Aliphatic estersThermogravimetric analysisDendrimersLithocholic acidHydrogen bondOrganic ChemistryDeoxycholic acidCholic acidBile acidschemistry.chemical_compoundchemistryDendrimerPolymer chemistryOrganic chemistryMoleculeHydroxymethylSteroidsThermal analysisPhysical and Theoretical ChemistryEuropean Journal of Organic Chemistry
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Bile acid–cysteamine conjugates: Structural properties, gelation, and toxicity evaluation

2011

Abstract Design, synthesis, and characterization of six novel bile acid–cysteamine conjugates together with investigation of their structural studies, gelation properties, and preliminary toxicity evaluation, are reported. Solid state properties of selected compounds were studied by means of X-ray diffraction and 13C CPMAS NMR spectroscopy. N-(2-thioethyl)-3α,7α,12α-trihydroxy-5β-cholan-24-amide was shown to exhibit (pseudo)polymorphism, and a single crystal structure of its non-stoichiometric hydrate is reported herein. Cholyl and dehydrocholyl derivatives bearing three functionalities in their steroidal backbone were shown to undergo self-assembly leading to gelation in certain organic so…

BALB 3T3 CellsMagnetic Resonance Spectroscopymedicine.drug_classCysteamineClinical BiochemistryCholic AcidBiochemistryBile Acids and SaltsInhibitory Concentration 50Micechemistry.chemical_compoundEndocrinologyX-Ray DiffractionmedicineAnimalsOrganic chemistryta116Molecular BiologyPharmacologyBile acidUrsodeoxycholic AcidOrganic ChemistryHydrogen BondingNuclear magnetic resonance spectroscopyFibroblastsAmidesCombinatorial chemistrychemistrySolid-state nuclear magnetic resonancePolymorphism (materials science)SolventsLithocholic AcidCysteamineHydrateSingle crystalDeoxycholic AcidConjugateSteroids
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Novel two-step synthesis of gold nanoparticles capped with bile acid conjugates

2008

Bile acids and their conjugates are physiologically important molecules. Syntheses and structure elucidation combined with investigation of properties and applications of bile acids and their derivatives are of academic interest. The concept of using bile acids and their conjugates in nanoscience is a novel idea, which opens up fascinating prospects. In this article, an easy and simple route for obtaining N-lithocholyl-L-(cysteine ethyl ester) (3), capable of effectively capping and stabilizing metal nanoparticles, is described. The whole synthetic route needs only two steps giving a moderate to good yield. The gold NPs are characterized by elemental analysis, UV spectroscopy, and TEM. Addi…

Lithocholic acidBile acidChemistryLigandmedicine.drug_classBioengineeringBiomaterialschemistry.chemical_compoundUltraviolet visible spectroscopyMechanics of MaterialsColloidal goldYield (chemistry)medicineMoleculeOrganic chemistryConjugateMaterials Science and Engineering: C
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3α,3′α-Bis(n-acetoxyphenylcarboxy)-5β-cholan-24-oic acid ethane-1,2-diol diesters (n = 2-4):13C NMR chemical shifts, variable-temperature and NOE1H N…

2000

Three novel bile acid-based molecular dimers, 3α,3′α-bis(n-acetoxyphenylcarboxy)-5β-cholan-24-oic acid ethane-1,2-diol diesters (n = 2–4), 1–3, were synthesized from lithocholic acid (3α-hydroxy-5β-cholan-24-oic acid) ethane-1,2-diol diester and isomeric n-acetoxybenzoyl chlorides (n = 2–4). Their cleft type conformational preferences were suggested theoretically by PM3 molecular orbital calculations. Molecular weights determined by the matrix-assisted laser desorption/ionization time-of-flight technique and 13C NMR chemical shifts of the 1–3 are also presented. Copyright © 2000 John Wiley & Sons, Ltd.

Lithocholic acidBile acidStereochemistrymedicine.drug_classChemical shiftDiolNuclear magnetic resonance spectroscopy of nucleic acidsGeneral ChemistryCarbon-13 NMRchemistry.chemical_compoundchemistryDesorptionmedicineGeneral Materials ScienceMolecular orbitalMagnetic Resonance in Chemistry
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Macrocycles prepared from lithocholic acid, piperazine and isomeric pyridine dicarboxylic acids and their selective affinities towards sodium and pot…

2001

Abstract Two novel macrocycles prepared from lithocholic acid, piperazine and pyridine dicarboxylic acids (2,6- and 3,5-isomers), have been characterized by 13C NMR and ESI–MS techniques. In case of the pyridine-2,6-dicarboxylate derivative, the molecular formula of the cycle was C59H87O6N3 (I), while the pyridine-3,5-dicarboxylate derivative (II) was a trimeric structure by molecular mass when compared with I. Furthermore, cycle I showed a special affinity towards potassium cation, while II possessed significant proton and sodium cation recognition properties.

Lithocholic acidChemistryPotassiumSodiumchemistry.chemical_elementBioengineeringCarbon-13 NMRPotassium CationMedicinal chemistryBiomaterialschemistry.chemical_compoundPiperazineMechanics of MaterialsPyridineOrganic chemistryDerivative (chemistry)Materials Science and Engineering: C
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Multinuclear magnetic resonance, electrospray ionization time-of-flight mass spectral and molecular modelling characterization of lithocholic acid am…

2003

1H, 13C and 15N NMR and electrospray ionization time-of-flight mass spectrometric characterizations of five lithocholate esters of piperazine diamides are described. Two of them are cholaphane-type cyclic structures esterified with 2,2′-bipyridine-4,4 ′ - and pyridine-2,6-dicarboxylic acid and the other three esters are open structures comprising two or four lithocholyl residues. The conformational preferences of the dimeric congeners were examined by using molecular modelling and variable-temperature 1H NMR techniques. Copyright © 2003 John Wiley & Sons, Ltd.

Lithocholic acidElectrospray ionizationchemistry.chemical_elementGeneral ChemistryCarbon-13 NMRNitrogenchemistry.chemical_compoundTime of flightPiperazinechemistryAmideProton NMROrganic chemistryGeneral Materials ScienceMagnetic Resonance in Chemistry
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